Energy recovery from lignocellulosic waste has been studied as an alternative to the problem of inappropriate waste disposal. The present study aimed at characterizing the microbial community and the functional activity of reactors applied to H2 production through lignocellulosic waste fermentation in optimized conditions. The latter were identified by means of Rotational Central Composite Design (RCCD), applied to optimize allochthonous inoculum concentration (2.32-5.68 gTVS/L of granular anaerobic sludge), pH (4.32-7.68) and Citrus Peel Waste (CPW) concentration (1.55-28.45 g/L). After validation, the conditions identified for optimal H2 production were 4 gSTV/L of allochthonous inoculum, 29.8 g/L of CPW (substrate) and initial pH of 8.98. In these conditions, 48.47 mmol/L of H2 was obtained, which is 3.64 times higher than the concentration in unoptimized conditions (13.31 mmol H2/L using 15 g/L of CPW, 2 gTVS/L of allochthonous inoculum, pH 7.0). Acetogenesis was the predominant pathway, and maximal concentrations of 3,731 mg/L of butyric acid and 3,516 mg/L of acetic acid were observed. Regarding the metataxonomic profile, Clostridium genus was dramatically favored in the optimized condition (79.78%) when compared to the allochthonous inoculum (0.43%). It was possible to identify several genes related to H2 (i.e dehydrogenases) and volatile fatty acids (VFA) production and with cellulose degradation, especially some CAZymes from the classes Auxiliary Activities, Glycoside Hydrolases and Glycosyl Transferase. By means of differential gene expression it was observed that cellulose degradation and acetic acid production pathways were overabundant in samples from the optimized reactors, highlighting endo-β-1,4-glucanase/cellulose, endo-β-1,4-xylanase, β-glucosidase, β-mannosidase, cellulose β-1,4-cellobiosidase, cellobiohydrolase, and others, as main the functions.Climate change increases risks to natural and human systems. Green infrastructure (GI) has been increasingly recognized as a promising nature-based solution for climate change adaptation, mitigation, and other societal objectives for sustainable development. Although the climate contribution of GI has been extensively addressed in the literature, the linkages between the climate benefits and associated co-benefits and trade-offs remain unclear. We systematically reviewed the evidence from 141 papers, focusing on their climate benefits, relevant co-benefits and trade-offs, and the GI types that provide such climate (co-)benefits. This study presents a comprehensive overview of the links between climate benefits, co-benefits and types of GI, categorized along a green-grey continuum so that researchers/practitioners can find information according to their topic of interest. We further provide an analysis of trade-offs between various GI benefits. https://www.selleckchem.com/products/pexidartinib-plx3397.html 'Bundles' of major co-benefits and trade-offs for each climate benefit can be identified with recommendations for strategies to maximize benefits and minimize trade-offs. To promote climate-resilient pathways through GI, it is crucial for decision-makers to identify opportunities to deliver multiple ecosystem services and benefits while recognizing disservices and trade-offs that need to be avoided or managed.The contamination of heavy metals in agricultural ecosystem is one of the most important problems in developing countries as Vietnam. In this study, we investigated the multi-element concentrations in soil, vegetables, soil-to-plant transfer factors and target hazard quotient (THQ) due to the consumption of heavy metals in Ho Chi Minh City, Vietnam. In general, the element concentrations in soil and plants were similar to different studies in the world and in the range of allowable values provided by WHO and the Ministry of Health of Vietnam. The transfer factors indicated the influence of element characteristics and plant genotypes on the accumulation and translocation of elements from soil to plants. It is found that I. batatas, B. alba, A, tricolor, O. basilicum, and B. juncea could be potential candidates for phytoremediation in soil contaminated of heavy metals. The results of individual and total THQ were below unity for Cr, Mn, Fe, Co, Zn, As, and Sb. The total THQ is in the range from 0.11 for R. sativus to 0.84 for B. alba with the average value of 0.43, in which Mn and As are the major contributions to the total THQ with the average values of 75% and 18%, respectively. The safety assessment based on national regulations and THQ indicated that the consumption of investigated vegetables poses no risk to the consumers.Contaminant levels are lower in Antarctica than elsewhere in the world because of its low anthropogenic activities. However, the northern region of the Antarctic Peninsula, is close to South America and experiences the greatest anthropogenic pressure in Antarctica. Here, we investigated, in two Antarctic Peninsula islands, intra and interspecific factors that influence the concentrations of 17 trace elements (TEs) in blood and feathers of three penguin species breeding sympatrically in relation to their trophic ecology assessed via a stable isotopic approach (C, N and S). Geographical location, foraging zone (δ13C and δ34S) and diet influences the interspecific difference, and sex and maturity stage diet influence the intraspecific difference of Pygoscelis penguins. Penguins from Livingston showed higher values (mean, ng. g-1, dry weight - dw) of Zn (103), Mn (0.3), and Fe (95) than those from King George Island (Zn 80, Mn 1.9, and Fe 11). Gender-related differences were observed, as males showed significantly higher values (mean, ng. g-1, dw) of Rb (3.4) and δ15N in blood of gentoo, and Ca (1344) in Adélie feathers. Chicks of gentoo and Adélie presented higher Zn, Mg, Ca, and Sr and lower 13C values in blood than adults. The highest concentrations (mean, ng. g-1, dw) of Cd (0.2) and Cu (26), and the lowest δ15N values were found in chinstrap. Geographical, intraspecific (i.e., ontogenetic and gender-related) and interspecific differences in feeding seemed to have influenced TE and stable isotope values in these animals. The TE bioaccumulation by penguins may have also been influenced by natural enrichment in environmental levels of these elements, which seems to be the case for Fe, Zn, and Mn. However, the high level of some of the TEs (Mn, Cd, and Cr) may reflect the increase of local and global human activities.